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KMID : 0861020080230040171
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Korea Journal of Herbology
2008 Volume.23 No. 4 p.171 ~ p.177
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Determination Method of Puerarin and Daidzin from Puerariae Radix by Reversed-Phase HPLC with Pulsed Amperometric Detection
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Chiao Chen-Ying
Kwon Ha-Jeong
Jeong Ji-Seon
Lee Je-Hyun
Hong Seon-Pyo
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Abstract
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Objectives: This study presents a reversed-phase high-performance liquid chromatography- pulsed amperometric detection(RP-HPLC-PAD) method for the determination of puerarin and daidzin in Puerariae Radix extract and Chinese medicinal preparations.
Methods: Chromatographic separation was performed using a 10% acetonitrile with a reversed-phase column(Unison UK-C18, I.D.; ). The analyses were detected by pulsed amperometric detector(PAD) in alkaline conditions by combining with post-column NaOH solution. Geniposide was used as an internal standard.
Results: The limit of detection(S/N=3) and the limit of quantification(S/N=10) were 0.025 ng, 0.075 ng for puerarin, and 0.05 ng, 0.15 ng for daidzin, respectively. The intra- and inter-day precisions(RSDs) were less than 6.5% and average recoveries of puerarin were 99.7-101.3% and those of daidzin were 101.0-102.8%.
Conclusions: According to above results, we developed a determination method for puerarin and daidzin in Puerariae Radix with high sensitivity and selectivitely.
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KEYWORD
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Reversed-phase high-performance liquid chromatography, Pulsed amperometric detection, Puerariae Radix, Puerarin, Daidzin, Chinese medicinal preparations
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